The Chan Zuckerberg Biohub and collaborators have introduced Ultrack and inTRACKtive, integrated AI-powered tools offering unprecedented speed and accuracy for cell tracking across 2D, 3D, and tissue scales. By combining segmentation and cell linking into a unified approach, Ultrack overcomes previous limitations in analyzing complex time-lapse imaging data, facilitating insights into embryonic development and disease. These publicly available tools promise to accelerate research in diverse biological disciplines by enabling high-fidelity cell trajectory reconstructions.