Significant progress marks regenerative medicine through advanced stem cell technologies. Researchers engineered aligned mesenchymal stem cell sheets that markedly increase secretion of reparative signaling proteins, enhancing tissue repair and immunoregulation prospects. A novel RNA switch integrating translational and post-translational controls achieves highly specific gene expression in target cell types. Additionally, production efficiency of cell sheets is optimized using thermo-responsive polymers tailored by brush length and density, promising improved cell-based therapies.