Innovations in spatial proteomics are enabling unprecedented molecular mapping within tissues, critical for understanding diseases like cancer. The GeoMx Discovery Proteome Atlas (DPA) expands protein profiling capabilities to over 1200 targets, including post-translational modifications, facilitating in situ analyses from tumor niches to single cells. Giotto Suite software streamlines multiomic spatial data analysis for researchers facing large, complex datasets. The Microscoop® platform offers unbiased, single-cell to subcellular protein isolation for mass spectrometry, overcoming limitations of targeted approaches. These integrative tools are poised to enhance biomarker discovery, spatial biology research, and translational applications across oncology, immunology, and neuroscience.