Cytosurge and Lexogen have launched a live-cell sequencing workflow integrating FluidFM cytoplasmic sampling and ultra-sensitive RNA library preparation to enable repeated transcriptomic profiling of the same living cells over time. This first-of-its-kind technology overcomes limitations of destructive sequencing by preserving cell viability, allowing researchers to study dynamic gene expression changes longitudinally. The breakthrough promises transformative applications in developmental biology, cancer research, and drug discovery, providing causal insights into cellular decision processes.