A new workflow using the NG-TEST Blood Culture PREP Kit and NG-TEST CARBA 5 enables accurate detection of five major carbapenemases directly from positive blood cultures in under 25 minutes. The approach targets a major bottleneck in antimicrobial stewardship: time-to-identification of carbapenem resistance mechanisms. For hospitals managing resistant Gram-negative infections, faster mechanism-level reporting may help clinicians narrow therapy earlier while supporting infection control decisions.