Boston Children’s Hospital and Harvard Medical School scientists have developed a fast, well-defined method to generate functional 3D vascular organoids from human induced pluripotent stem cells. Their approach simultaneously activates two transcription factors, ETV2 and NKX3.1, driving coordinated differentiation of endothelial and mural cells, the key components of blood vessels. These organoids demonstrate self-assembly, mature vascular networks, and successful integration with host vasculature upon implantation. This method accelerates development timelines and provides precise lineage control, representing a powerful platform for modeling vascular biology, tissue engineering, and regenerative medicine applications.