Integrated DNA Technologies (IDT) introduced a workflow aimed at improving CRISPR off-target site nomination, using UNCOVERseq to streamline the jump from high-throughput discovery to targeted confirmation. In a Nature Communications benchmarking study, UNCOVERseq reported an increased number of high-confidence sites and nearly 98% analytical sensitivity. IDT said the method can be used in cell lines and under promiscuous editing conditions, designed to generate data in a more native cellular context. The update supports IDT’s stated strategy to build an end-to-end gene editing services capability by addressing gaps in standardized off-target safety assessment. —