An observational evaluation in Colombia assessed point-of-care rapid tests for Chagas disease in pregnant women and reported high sensitivity and specificity across multiple products when compared with the country’s official serological algorithm. The study’s structure is especially relevant for implementation because it tests real-world operational performance rather than only lab settings. The result is supported by point estimates of sensitivity in the high range and specificity approaching or reaching 100% for several tests, with the top performer showing the strongest single-test performance. It also examined combined diagnostic algorithms in serial and parallel configurations. For biotech and diagnostics developers, the adoption signal is that these rapid tests can reduce lab dependence in antenatal settings, potentially improving timing of intervention for congenital risk reduction. The item credits DNDi and the Colombian National Institute of Health among the collaborators, emphasizing cross-stakeholder validation work required for adoption.