Researchers at VIB and VUB have introduced an orthotopic lung adenocarcinoma model paired with advanced single-cell sequencing to map the immune microenvironment with greater patient relevance. The platform is designed to capture how tumor location shapes immune composition and activity inside lung tumors. By integrating the improved model with high-resolution immune profiling, the team reports one of the most detailed immune landscape characterizations in lung cancer to date. The dataset and platform could be used to refine immunotherapy hypotheses and improve interpretation of immune-tumor interactions when evaluating therapeutic strategies.