Researchers at Tulane University have developed ActCRISPR-TB, a novel CRISPR-Cas12a assay enabling rapid, sensitive detection of Mycobacterium tuberculosis DNA from a simple tongue swab. This non-invasive “one-pot” test offers results comparable or superior to standard sputum tests within an hour, facilitating diagnosis especially in resource-limited settings and among patients unable to provide sputum samples. The assay integrates isothermal amplification and CRISPR detection, representing a significant advancement in tuberculosis screening and control efforts.