Ultima Genomics pioneered paired-plus-minus sequencing (PPM-seq), an enhanced duplex sequencing method delivering tenfold higher yield and superior accuracy in DNA variant detection. PPM-seq doubles sequencing accuracy by analyzing both DNA strands simultaneously, achieving variant error rates as low as 7.98×10^−8. This innovation lowers the coverage requirements for whole-genome sequencing, significantly advancing applications such as minimal residual disease detection and liquid biopsy diagnostics.<BR><BR>These improvements could reshape clinical oncology genomics by enabling more precise, cost-effective molecular profiling of tumors and circulating DNA with standard sequencing protocols.