Researchers deployed nanopore sequencing on plasma cell‑free DNA from critically ill patients to capture simultaneous tissue‑of‑origin signals and detect pathogenic DNA. The study demonstrated that long‑read nanopore approaches can provide both host tissue fingerprinting and strain‑level pathogen identification from a single minimally invasive sample, potentially enabling faster diagnostics in critical care. Investigators emphasized the method’s real‑time sequencing and long‑read advantages for resolving complex cfDNA signals. Clinical translation will require validation across larger cohorts, workflow standardization, and regulatory clearance for diagnostic use.