Swiss company 4bases and the University of Florence launched clinical pilot studies of a nanopore sequencing‑based cytogenetics workflow, KaryoSolver, in two Italian hospitals. The pilots aim to validate rapid detection of copy‑number variants (CNVs) in real‑world samples and benchmark the method against array‑comparative genomic hybridization. The workflow uses PromethIon throughput and a read‑count CNV approach to reduce turnaround to roughly 24 hours and enable higher multiplexing per run, a potential time and cost advantage for diagnostic cytogenetics.