Researchers led by Clowsley, Meletiou and Janicek applied MINFLUX super‑resolution microscopy to resolve the three‑dimensional subunit architecture and orientation of the cardiac ryanodine receptor (RyR) in living cells. The work provided molecular-level imaging of excitation–contraction coupling machinery, revealing structural details that were previously obscured by resolution limits. The 3D MINFLUX maps may inform structural models of RyR regulation and dysfunction in cardiomyopathies and could guide future efforts to target RyR pharmacologically or with gene-based interventions.