A new workflow enables rapid detection of major carbapenemases directly from positive blood cultures in under 25 minutes using the NG-TEST Blood Culture PREP Kit and NG-TEST CARBA 5. The approach targets five major carbapenemase types, aiming to shorten the time between culture positivity and actionable antimicrobial guidance. Speed matters in bloodstream infections where early effective therapy can be life-saving. For clinical microbiology programs, the study also supports a trend toward consolidating specimen prep and molecular/biochemical detection into faster, lab-integrated processes.