Scientists at Chan Zuckerberg Biohub developed a mass spectrometry–based method to spatially map the subcellular localization of proteins, revealing dynamic relocalization during viral infection. This technique allows enrichment of organelles followed by quantification of constituent proteins, uncovering protein redistribution associated with ferroptotic cell death. This approach enhances understanding of protein function within cellular compartments, critical for elucidating disease mechanisms and cellular responses to pathogens.