The 1000 Genomes Project Long-Read Sequencing (1KGP-LRS) Consortium has expanded its scope by incorporating PacBio sequencing in addition to Oxford Nanopore Technologies data. Led by researchers at the University of Washington, the project aims to produce comprehensive long-read DNA and RNA sequencing across diverse human populations. This expansion includes generating full-length isoform RNA sequencing data for about 1,000 samples, enabling deeper insights into isoform diversity and structural genetic variation. The consortium’s publicly released datasets are anticipated to serve as a critical resource for genetics and genomics research communities.