German researchers from the University of Cologne have optimized the CUT&Tag assay by reducing salt concentrations in buffers, overcoming prior limitations preventing measurement of transcription factor binding. Their innovative method, "DynaTag", allows detection of transient protein-DNA interactions, demonstrated with c-Myc factor activity changes post-chemotherapy in small-cell lung cancer models. This breakthrough advances chromatin accessibility profiling from histone modifications to dynamic transcription factor occupancy, enabling single-cell and potential spatial analyses. The study, published in Nature Communications, refines and expands CUT&Tag’s applications in gene regulation research.