Researchers have developed novel AI-based methods to design small enzymes tailored for bioprocessing and pioneered AI-assisted gene editing precision. ProtGPT2, a language model for proteins, generated structurally stable small malate dehydrogenase variants with active sites similar to natural enzymes, opening avenues for efficient bioprocess catalysts. Complementing this, an AI tool named Pythia predicts DNA repair outcomes following CRISPR cuts, facilitating precise genome integrations across species. These advances mark significant progress toward safer, more effective gene editing and protein engineering applications in biotechnology.