Innovative approaches in stem cell science promise to transform regenerative therapies. Researchers engineered aligned mesenchymal stem cell sheets, significantly boosting protein secretion relevant for tissue repair and immune regulation. A novel technique generates lung alveolar epithelial-like cells from mouse fibroblasts in just 7 to 10 days without stem cell intermediates, drastically shortening production time. Additionally, the application of aligned stem cell sheets advances treatments targeting chronic and acute diseases, potentially improving clinical outcomes across diverse regenerative medicine domains.