A new workflow described rapid detection of five major carbapenemases directly from positive blood cultures in under 25 minutes using the NG-TEST Blood Culture PREP Kit paired with the NG-TEST CARBA 5. The approach aims to shorten time to targeted antimicrobial decisions by reducing reliance on slower confirmatory testing. If validated broadly, the platform could improve stewardship during bloodstream infections where treatment choices depend on early resistance detection. The main operational benefit highlighted is speed without extending specimen turnaround.