A team led by Ma and Kim published a dilution‑and‑delay (DnD) susceptibility assay in Nature Communications that detects low‑frequency or time‑dependent antibiotic resistance missed by standard tests. The high‑resolution, high‑throughput method exposes ‘hidden’ resistances and heteroresistant subpopulations, offering a path to more accurate laboratory susceptibility profiles and guiding therapy choices. Clinical microbiology labs and antibiotic stewardship programs could adopt DnD to reduce treatment failures and better inform drug development against refractory pathogens.
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