A suite of innovative spatial biology tools is revolutionizing how researchers visualize and analyze complex molecular landscapes within tissues. The Giotto Suite software facilitates integration and interpretation of spatial multiomic data, simplifying workflows for cancer and neurodegenerative disease studies. Parallel advancements by Bruker’s GeoMx Discovery Proteome Atlas offer high-plex protein profiling of tissue architecture, uncovering disease mechanisms beyond RNA analysis. Meanwhile, Stellaromics’ Pyxa system enables three-dimensional spatial transcriptomics of thick tissue sections, capturing cellular contexts previously inaccessible. These cutting-edge technologies are driving new avenues for biomarker discovery and precision medicine.