TB-Profiler received a major upgrade that expands its genomic reference capacity to improve drug-resistance prediction. The tool’s newest version incorporates 171,000 genomes to sharpen susceptibility and resistance calls for tuberculosis. The update is intended to reduce uncertainty in rapid molecular diagnostics, where accurate interpretation of resistance mutations is critical for choosing effective regimens and avoiding treatment failure. For clinical laboratories and public health programs, the practical value is increased resolution and potentially better performance across more geographic strains as reference diversity grows.