Cutting-edge tools in genomics and molecular biology continue to enable transformative advances in medicine. Novel methods for accurate CRISPR off-target prediction have improved with consideration of genetic variants, addressing genome-editing specificity challenges. Spatial omics combined with AI analytics is revolutionizing single-cell and tissue-level profiling, enhancing insights into tumor heterogeneity and patient prognosis. Furthermore, new photonic biosensors and optical technologies promise increased diagnostic sensitivity, shaping the next generation of precision medical interventions.