Efforts to deepen and broaden long-read sequencing in the landmark 1000 Genomes Project have gathered momentum. The consortium has incorporated Pacific Biosciences’ technologies alongside Oxford Nanopore sequencing, aiming to comprehensively characterize genome structural variation and isoform diversity with full-length RNA sequencing. This multi-institutional collaboration will generate a publicly accessible dataset, offering unprecedented resolution that is expected to fuel research into human genome variation and its implications in health and disease.