An engineered Bacillus subtilis strain (WBSS142I4PA) achieved record extracellular invertase production, reaching 568.86 U/mL. The enzyme output targets conversion of soybean raffinose into prebiotic melibiose, aligning bioprocess performance with functional food goals. The report’s emphasis on extracellular production matters for downstream processing economics, since secreted enzymes can reduce cell-lysis steps and purification complexity compared with intracellular targets. While this is a production/biomanufacturing advance rather than a clinical pipeline event, it directly affects how quickly and cost-effectively fermentation-derived ingredients can scale for commercial use.