A scientific-integrity investigation expanded significantly on concerns about antibody validation image manipulation. Reese Richardson and collaborators reported finding more than 18,000 questionable images linked to antibodies sold by 15 companies, following prior work that implicated thousands of altered “validation images” and costing researchers time and money when antibodies fail. The update named major vendors including Thermo Fisher Scientific, with a large share of flagged images, and others such as G-Biosciences, LSBio, and Origene. The findings are likely to intensify pressure for stronger third-party validation standards and improved catalog transparency for researchers who depend on antibody performance for diagnostics and reproducible experiments.