Scientists at the University of Cologne have innovated on the CUT&Tag chromatin accessibility assay by optimizing buffer salt concentrations, enabling the detection of transcription factor binding previously unresolvable due to assay conditions. This advance, called "DynaTag," allows researchers to monitor transient transcription factor occupancy, including oncogenic factors like c-Myc, with single-cell capability and integration with RNA-seq. Published in Nature Communications, this breakthrough enhances the assay’s use in epigenomics and cancer research, offering new insights into transcriptional regulation dynamics under chemotherapy and other conditions.