Researchers unveiled CRISPR PRO‑LiveFISH, a multiplexed live‑cell imaging approach that visualizes chromatin dynamics and promoter–enhancer interactions at multiple non‑repetitive loci. Published in Nature Biotechnology, the method overcomes longstanding limitations in live genome imaging by enabling tracking of dynamic three‑dimensional DNA contacts with high spatial resolution. The technique allows interrogation of enhancer behavior in real time, providing a tool to test how epigenetic states and perturbations alter transcriptional regulation. Authors demonstrate applications across cell types and manipulation conditions, positioning CRISPR PRO‑LiveFISH as a new platform for mechanistic studies of gene regulation and drug effects on chromatin architecture. The advance promises utility for both basic chromatin biology and applied discovery workflows focused on regulatory element targeting.