The convergence of CRISPR technology and artificial intelligence is accelerating viral pathogen detection and genome editing precision. A novel CRISPR-based assay enables rapid identification of BK and JC viruses. Concurrently, the deep learning platform Pythia predicts optimal DNA repair templates for CRISPR-Cas9 gene editing with high accuracy, improving editing outcomes in diverse cellular contexts. These innovations enhance disease diagnostics and therapeutic genome engineering.