A quasi-experimental hospital study found that substituting 0.5% chlorhexidine gluconate for normal saline during periurethral cleansing at catheter insertion reduced catheter-associated urinary tract infections by 62.6%. The analysis is focused on a straightforward procedural swap during routine care. The magnitude of reduction makes it relevant for antimicrobial stewardship strategies and health system infection-prevention protocols, especially in catheter-dependent workflows. The study’s hospital-based design suggests feasibility for implementation without requiring new devices or major infrastructure. For biotech stakeholders in infection control and clinical product development, the results add evidence that antiseptic regimen optimization can produce large real-world infection impacts.