Researchers constructed a genome-scale CRISPRi perturbation atlas in human induced pluripotent stem cells, pairing large-scale CRISPR interference with single-cell transcriptomics to map gene regulatory function across pluripotency. The work was reported in Nature Biotechnology, detailing a transcriptional regulatory map generated by CRISPR screening. The atlas provides a cell-state resolved view of how gene perturbations affect expression programs, which can help identify regulators that maintain or destabilize pluripotent states. “CRISPRi perturbations combined with single-cell transcriptomics” refers to turning genes down rather than cutting DNA, then reading out the resulting expression changes at single-cell resolution. For regenerative medicine and cell therapy developers, the dataset supports better-informed reprogramming and differentiation engineering by revealing which regulatory levers drive specific pluripotent transcriptional outputs.