New platforms for spatial proteomics and high-plex protein profiling are expanding the depth and resolution of in situ molecular analysis. Bruker’s GeoMx Discovery Proteome Atlas sets a new standard with over 1,200 curated protein targets, enabling detailed analysis of tumor microenvironments, immune cell niches, and neural tissue with multiplexing that includes post-translational modifications. Spatial biology is further revolutionized by tools like Stellaromics’ Pyxa, which enables three-dimensional spatial transcriptomics in thick tissue sections, preserving intact architecture and cell-to-cell interactions. These advancements promise to drive biomarker discovery, clinical translation, and a refined understanding of disease biology.