Innovations in imaging are reshaping research capabilities across biological sciences. Novel chip-based super-resolution microscopy enables label-free visualization at unprecedented resolution, complemented by confocal squared spinning-disk microscopy enhancing tissue imaging fidelity. Developments in wide-field single-molecule fluorescence lifetime imaging and advanced MRI techniques, such as enhanced 3D-NEVERview for nasopharyngeal carcinoma, improve diagnostic precision and therapeutic planning. These technologies promise augmented insights into cellular and molecular processes, driving forward biomedical discovery and clinical care.