Researchers at the University Medical Center Utrecht have successfully utilized mitochondrial base editing (mtBE) to correct pathogenic mitochondrial DNA mutations in patient-derived cell models. This CRISPR-independent approach enables precise cytosine-to-thymine conversions within mitochondrial genomes, addressing a long-standing challenge in mitochondrial gene therapy. The study demonstrates restored mitochondrial function, marking a critical advance toward therapeutic applications for mitochondrial diseases.