Emerging innovations in biomanufacturing are leveraging automation, AI, and advanced modeling to streamline processes and reduce costs. Notably, new mathematical models for in vitro transcription enable fed-batch mRNA production, enhancing efficiency and cost-effectiveness compared to traditional batch methods. Concurrently, hybrid machine learning approaches combined with thermodynamic modeling and robotics optimize mammalian cell culture media formulation, improving protein yields while ensuring solubility constraints. Additionally, inline buffer preparation systems are gaining traction in bioprocessing for reducing space, labor, and costs with optimized stock solutions. Collectively, these technologies mark significant progress toward smarter, scalable biomanufacturing.