Researchers have pioneered innovative stem cell technologies to enhance tissue repair and regeneration. Aligned mesenchymal stem cell sheets significantly amplify therapeutic protein secretion. Rapid conversion of fibroblasts to lung epithelial-like cells without stem cell intermediates reduces differentiation time to 7–10 days. Techniques to boost platelet production from iPSC-derived megakaryocytes via microtubule destabilization show promise. Such advances pave the way for improved regenerative therapies and personalized treatment strategies.