Oxford Nanopore Technologies introduced a 24-hour rapid whole-genome sequencing (WGS) workflow specifically targeting rare disease research, particularly in critical care scenarios like neonatal and pediatric intensive care units. The protocol includes DNA extraction, fragmentation, library preparation, and sequencing using PromethIon flow cells with real-time basecalling and variant analysis. While designed for research use, the technique aims to facilitate ultra-rapid genetic insights to inform patient care in critical settings. The workflow supports capabilities such as methylation analysis and phased variant detection, representing a significant advancement in genomic diagnostics.