The 1000 Genomes Project continues to deepen insights into human genome complexity. The Long-Read Sequencing Consortium, led by the University of Washington, expanded its scope by integrating PacBio sequencing alongside nanopore technology to produce nearly complete human genome assemblies. This work closes many assembly gaps and reveals extensive structural variants across diverse populations, advancing the understanding of genome variation and evolution. These data serve as valuable resources, enhancing variant detection fidelity and providing references for biomedical research.