A computational platform rapidly generates vascular tree networks for organ-scale 3D bioprinting, achieving a 230-fold speed improvement over prior methods. Developed vasculature geometries have been tested in bioreactors, confirming enhanced perfusion and cell viability in printed tissue constructs. This work, published in Science, addresses a longstanding bottleneck in biofabrication by enabling organ-spanning synthetic vasculature essential for complex tissue and organ manufacturing.