The 1000 Genomes Project Long-Read Sequencing Consortium expanded its scope by integrating PacBio data alongside Oxford Nanopore sequencing, aiming to sequence the entire 1000 Genomes cohort with both technologies. This effort improves resolution of structural variants and isoform diversity critical for genomics research. PacBio contributes full-length isoform RNA-seq for approximately 1,000 samples using its Kinnex kit and Revio platform, while bioinformatics expertise supports complex transcriptomic analyses. These advances promise a comprehensive resource for human genetic variation studies.